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. 2013 Jan 25;304(7):H966–H982. doi: 10.1152/ajpheart.00883.2012

Fig. 3.

Fig. 3.

Autologous mitochondrial transplantation ameliorates myocardial injury and enhances regional function. A: representative photograph of RI-vehicle and RI-mitochondria hearts stained with 1% triphenyltetrazolium chloride (TTC) after 28 days of recovery. White areas show myocardial necrosis. Brick red areas show viable tissue. Scale in mm is shown. B: area at risk, the area subjected to regional ischemia and infarct size as determined by TTC staining in RI-mitochondria and RI-vehicle hearts after 2 h of recovery and after 28 days of recovery. Results are shown as means ± SE; n = 7–8 for each group. **Statistical differences at P < 0.05 vs. RI-vehicle. C and D: representative 2-dimensional (2D) short-axis images of left ventricular diastole and systole at the midportion of the left ventricle are shown for RI-vehicle (C) and RI-mitochondria (D) hearts following 28 days of recovery. ECG wave forms are shown in green at the bottom of each image. The arrows in C indicate regional hypokinesis within the ischemic zone in RI-vehicle heart. Comparative M-mode is indicated with arrows. Hypokinesis was not observed in RI-mitochondria hearts. Representative transthoracic 2D short-axis videos are provided as Supplemental Videos S1 and S2 (available on the AJP-Heart and Circulatory Physiology web site). E and F: CK-MB (ng/ml; E) and cTnI (ng/ml; F) were measured serially on days 1 and 3 following myocardial ischemia in serum from RI-vehicle (black bars) and RI-mitochondria hearts (open bars). Results are shown as means ± SE; n = 7–8 for each group. **Statistical differences at P < 0.05 vs. RI-vehicle. G: total tissue ATP content (μmol/g dry weight) in the area at risk of RI-vehicle and RI-mitochondria hearts at 21 days of recovery. All results are shown as means ± SE; n = 4 for each group. **Significant differences at P < 0.05 vs. RI-vehicle. H and I: TUNEL (positive cell nuclei/1,000 cells; H) and caspase-3 activity (active units: pmol DVED-pNA·μg−1·min−1; I) in RI-vehicle and RI-mitochondria at 28 days of recovery are shown. Results are shown as means ± SE; n = 7–8 for each group. **Statistical differences at P < 0.05 vs. RI-vehicle.