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. 2013 Feb 1;304(7):H903–H915. doi: 10.1152/ajpheart.00567.2012

Fig. 7.

Fig. 7.

Regulation of myocardial mitochondrial topoisomerase activity. A: H2O2 increases mitochondrial topoisomerase-dependent DNA cleavage. Mitochondria were preincubated in the absence or presence of 60 μM H2O2 prior to the addition of DNA. *P < 0.05 compared with respective no mitochondria/H2O control; #P < .0.05 compared with +mitochondria/H2O. B: dephosphorylation alters mitochondrial topoisomerase DNA cleavage activity. No Mito: no extract; +Mito: untreated mitochondria; CIP trt, mitochondria treated with 1 U calf intestinal phosphatase for 30 min at 37°C before DNA was added. Values are means ± SE and are normalized to no Mito or no Mito/H2O; *P < 0.05 compared with No Mito; #P < 0.05 compared with untreated mitochondria.