Figure 7.

Leucine-rich repeat-containing G-protein coupled receptor 5 does not promote ES cell proliferation. (A) qRT-PCR confirmation of LGR5 knockdown in ES cell lines transduced with LGR5-targeted shRNAs (shNS = non-silencing control). (B) Confirmation of LGR5 over-expression in A673 cells transduced with LGR5 over-expression construct (Empty, empty vector control). (C) ES cell expansion was measured by MTS assay and was not affected by LGR5 knockdown. (D) LGR5 over-expression did affect cell growth in A673 cells. (E) Parental cell growth was not affected by the addition of WNT3A and/or RSPO2 to standard culture conditions. All data shown is from two independent experiments and error bars are SEM.