Fig. 2.

Basal expression profile of the α₁- and α₂-isoforms of the Na,K-ATPase and Na/Ca-exchanger (NCX). Representative immunoblots and relative protein expression for α₁, α₂, NCX, and GAPDH or calsequestrin (CSQ) in the heart (A), aorta (B), brain (C), and kidney (D). Relative protein expression was determined by densitometry and normalized to the abundance of GAPDH or CSQ. The data represent an arbitrary ratio of protein expression in knockout mice compared with expression in control mice. The data were obtained from 3 independent experiments and are displayed as arbitrary units ± SE, n = 3. *P = 0.008.