Table 1.

NMR Restraints and Structural Statistics for the Best 20 Structures of WT and T170E Mutant of Human CARD2

Restraints and Statistics	WT	T170E Mutant
Total number of restraints	2,900	2,212
NOE restraints	2,646	1,944
Unambiguous	2,173	1,636
Intraresidue	945	822
Sequential	445	345
Short-range	274	217
Medium-range	128	66
Long-range	381	186
Ambiguous	473	308
Dihedral angle restraints	174	182
Hydrogen bond restraintsa	80	86
Structure statisticsb
NOE violations > 0.5 Å	0%	0%
Dihedral violations > 5°	0%	0%
RMSD from average structurec,d
Backbone (N, Cα, C) (Å)	0.44 ± 0.08	0.44 ± 0.09
Heavy atoms (Å)	0.97 ± 0.08	0.93 ± 0.08
Ramachandran Statisticse
Most favored region (%)	90.7	91.1
Additionally allowed (%)	8.6	8.6
Generously allowed (%)	0.3	0.1
Disallowed (%)	0.3	0.2

^aHydrogen bond restraints were H^N-O distance of 1.8–2.3 Å and an N-O distance of 2.8–3.3 Å.

^bStructural characteristics for the final ensemble of 20 water-refined structures.

^cRMSD of the mean structure from individual structures in the ensemble.

^dRMSD for residues 95–190 shown.

^eRamachandran plot data shown for residues 95–1190.