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. Author manuscript; available in PMC: 2013 Apr 15.
Published in final edited form as: J Membr Biol. 2012 Jun 30;245(0):243–253. doi: 10.1007/s00232-012-9445-3

Fig. 1.

Fig. 1

Schematics of time-lapse imaging using a dual-fluorescence excitation mode. a Whole-field excitation (vertical, blue, dashed arrows) of the cell pair with two patch pipettes. Emitted light of dye (yellow) in the pipette, and cell 1 creates a large background of scattered light around the cell pair. b The same as in a but excitation light is focused only to cell 2. This eliminates excitation of dye in cell 1 and the pipette attached to it. c Molecular formulas of positively charged fluorescent dyes, EAM-1 and EAM-2