. Author manuscript; available in PMC: 2013 Apr 15.

Published in final edited form as: Biochem Biophys Res Commun. 2001 Jun 15;284(3):596–601. doi: 10.1006/bbrc.2001.5027

Table 1.

Location of mutations in the TMs and detection by ELISA of HA-tagged A₃ human A₃ AR mutants expressed on the surface of COS-7 cells. Data are presented as means ± standard deviation of eight independent determinations.

Construct	Location (TM.residue)^a	Expression (% of WT)^c
N30A	1.50	110 ± 5
C88F	3.30	127 ± 18
D107N	3.49^b	138 ± 32
D107K	3.49^b	112 ± 16
D107R	3.49^b	142 ± 39
R108K	3.50^b	107 ± 5
R108A	3.50^b	98 ± 4
R108N	3.50^b	121 ± 23
R108E	3.50^b	79 ± 26
R108H	3.50^b	29 ± 22
Y109F	3.50^b	137 ± 13
D107K/R108K	3.49/3.50^b	133 ± 25
D107K/R108E	3.49/3.50^b	126 ± 34
A229E	6.34	96 ± 7
Y282F	7.53	163 ± 41

using notation of van Rhee and Jacobson (26). Homologous sites in other GPCRs were mutated resulting in CAMs (13, 24, 25, 34, 35).

DRY motif.

Expression level as percentage of HA-tagged A₃ WT (100 %).