Skip to main content
. Author manuscript; available in PMC: 2013 Aug 21.
Published in final edited form as: Immunity. 2013 Feb 7;38(2):373–383. doi: 10.1016/j.immuni.2012.10.021

Figure 4. Memory phenotype T cells in adults have the typical memory features and are absent in cord blood.

Figure 4

(A) Contour plots showing IFN-γ response of HIV-1- and PB1-specific T cells to stimulation by PMA and ionomycin. Experiments were repeated twice using blood from 2 different individuals. (B) Gene expression of tetramer negative and HIV-1 tetramer labeled cells (TetNeg mem n = 48, TetNeg naïve n = 46, HIV-1 mem n = 53, HIV-1 naïve n = 52). Heatmap summarizes the fraction of cells expressing a particular gene out of the total number of cells assayed. The genes have been grouped by whether they associate with memory (top) or naïve T cells (bottom) and then ordered by ascending p value that compares the differences between HIV memory and HIV naïve T cells. (C) TCRβ sequencing of tetramer labeled CD45RO+ and CD45RO− cells. Each pie chart represents TCR sequences from one individual. Dark gray: the fraction of cells expressing a TCR identical to that of another cell. Light gray: cells expressing unique TCRs. HIV-1 and HSV-specific T cells were obtained from individuals negative for these infections. (D) HA- and HIV-1-specific T cells in adult PBMC (top) or cord blood (bottom) were identified by tetramer staining followed by magnetic bead enrichment (left) and anti-CD45RO surface staining (right). Data is representative of two adults PBMCs and two cord bloods assayed in parallel (See also Fig. S4).