Figure 2. GVHD decreases recovery of vaccine-responding T cells.

Mice were transplanted per Figure 1A. (A) A sample flow cytometry gating schema on (top) a F1 recipient transplanted with syngeneic CD45.1⁺CD45.2⁺ F1 bone marrow and DLI, followed by CD45.1^-CD45.2⁺ Matahari splenocyte infusion, as well as (bottom) a F1 recipient transplanted with allogeneic CD45.1⁺CD45.2^- B6 bone marrow and DLI, followed by CD45.1^-CD45.2⁺ Matahari splenocyte infusion. The CD45.2⁺ Vβ8.3⁺ fraction in both BMTs represents the HY-reactive T cells from the Matahari infusion in the recipient spleen. (B) Absolute numbers of CD4⁺Vβ6⁺CD45.2⁺ Marilyn T cells in the host spleen were enumerated by flow cytometry 3, 5 and 7 days (days +31, 33 and 35 post-BMT) after adoptive transfer without concurrent male DC vaccination, or (C) with male DC vaccination, 4-9 mice/group, pooled from three independent experiments. (D) Absolute numbers of CD8⁺Vβ8.3⁺CD45.2⁺ Matahari T cells in the host spleen were enumerated by flow cytometry 3, 5 and 7 days (days +31, 33 and 35 post-BMT) after adoptive transfer without concurrent male DC vaccination, or (E) with male DC vaccination, 3-9 mice/group. * = p<0.05, pooled from three independent experiments.