Figure 5. Administration of perforin-deficient DLI attenuates GVHD-induced apoptosis of TCRTg CD8+ T cells in spleens and lymph nodes of alloBMT recipients.
(A) C3H.SW recipients received TCD B6 bone marrow on day +0 followed by 30 × 106 DLI from B6 (wild type, WT), B6 FasL-/- (GLD), B6 TRAIL-/-, or B6 prf1-/- (perforin) donors on day +14. On day +28, 10 × 106 CFSE-labeled Matahari T cells were adoptively transferred and all recipients concurrently received B6 male DC vaccine. After 7 days (day +35), nonproliferated CFSE+ Annexin V+ cells were enumerated in (B) spleens and (C) pooled axillary, inguinal and cervical lymph nodes from alloBMT recipients, 5-10 mice/group, * = p < 0.05, data pooled from two independent experiments.