Table 7. .
Confirmation of Selected BeadChip Gene Expression Results
|
Gene |
EGF | EGF | BPE | BPE | EGF + BPE | EGF + BPE |
|
Array |
qPCR |
Array |
qPCR |
Array |
qPCR |
|
| Upregulation | ||||||
| LAMA3 | 11.2 | 8.2 | 9.6 | 5.7 | 10.3 | 4.9 |
| TIMP1 | 11.7 | 13.6 | 4.8 | 4.9 | 8.4 | 6.9 |
| Downregulation | ||||||
| KRT10 | 10.6 | 10.8 | 10.2 | 13.7 | 12.4 | 16.3 |
| SPRR3 | 14.0 | 10.5 | 1.6 | 3.0 | 22.9 | 11.4 |
The expression of selected genes that were shown to be up- or downregulated by growth factors in human meibomian gland epithelial cells by using Illumina BeadChips (“Array”), were reexamined with qPCR procedures. LAMA3, Laminin, α3; TIMP1, TIMP metallopeptidase inhibitor 1; KRT10, keratin 10; SPRR3, small proline-rich protein 3.