Table 1.
Gene | Primer sequence | Amplified length |
---|---|---|
β-actin | 5′-ACC TTC TAC AAT GAG CTG CG-3′ (sense) | 148 bp |
5′-CCT GGA TAG CAA CGT ACA TGG-3′ (antisense) | ||
ALP | 5′-GAT GTG GAG TAT GAG AGT GAC G-3′ (sense) | 142 bp |
5′-GGT CAA GGG TCA GGA GTT C-3′ (antisense) | ||
COL1A2 | 5′-AGG ACA AGA AAC ACG TCT GG-3′ (sense) | 146 bp |
5′-GGT GAT GTT CTG AGA GGC ATA G-3′ (antisense) | ||
OC | 5′-CAG CGA GGT AGT GAA GAG AC-3′ (sense) | 144 bp |
5′-TGA AAG CCG ATG TGG TCA G-3′ (antisense) |
ALP: alkaline phosphatase, COL1A2: collagen 1A2, OC: osteocalcin.