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. 1989 Feb;9(2):578–585. doi: 10.1128/mcb.9.2.578

The adenovirus-inducible factor E2F stimulates transcription after specific DNA binding.

A S Yee 1, P Raychaudhuri 1, L Jakoi 1, J R Nevins 1
PMCID: PMC362634  PMID: 2523514

Abstract

The promoter-specific factor E2F interacts with critical regulatory sequences within the adenovirus E2 promoter. In addition, the level of active factor increases markedly during a virus infection, dependent on E1A function and coincident with the trans activation of E2 transcription. We have purified the E2F factor through a combination of standard biochemical procedures and DNA affinity chromatography. The purified factor was a single polypeptide of 54,000 molecular weight, as determined by UV crosslinking and renaturation of gel-fractionated protein. Addition of affinity-purified factor to an in vitro transcription system resulted in stimulation of transcription from a promoter containing two E2F-binding sites but not promoters lacking binding sites. We thus conclude that E2F is indeed capable of stimulating transcription once it has bound to the promoter.

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Selected References

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