Figure 2.

Calcium-dependent isoforms of PKC are necessary for glycine-induced presynaptic potentiation. A, B, From representative experiments, top traces show the average baseline EPSC response (left), potentiated response in glycine (middle), and response with strychnine application (right). Bottom, Plots of normalized EPSC amplitudes as a function of time. At t = 0 s, glycine wash-in was initiated, eliciting potentiated EPSC amplitudes in wild-type (wt) controls (A) but not littermate PKCαβ dko animals (B). C, Cumulative histograms of normalized amplitudes of the potentiated response in glycine for wild-type (black), PKCα (green), PKCβ (red), and dko (purple) calyces. D, Percentage ± SEM EPSC enhancement in glycine for wild-type and PKC ko groups. EPSCs were normalized to baseline before determining the amount of synaptic enhancement. *p < 0.05.