Figure 4.

Glycine elevates presynaptic calcium levels to a similar extent in wild-type (wt) and PKCα/β dko animals. The effects of glycine on the resting calcium concentration and on calcium transients after single stimuli at the calyx of Held were measured for wild-type mice (black), wild-type mice in the presence of the glycine receptor antagonist strychnine (blue), and PKCα/β dko (purple) mice. Representative calcium signals from individual experiments (A), the average time courses of glycine on the resting presynaptic calcium concentration (B), and the average effect of glycine on the resting calcium concentration and the amplitude of presynaptic calcium signals evoked by single stimuli (C) are shown. *p < 0.05.