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. 2013 Mar 28;54(3):2367–2372. doi: 10.1167/iovs.12-11013

Figure 1.

Figure 1

EMP2 increases VEGF protein expression. Steady-state protein levels of EMP2 were measured by Western blot analysis in ARPE-19 cells and ARPE-19/EMP2 cells. EMP2 overexpression increased VEGF expression by 1.5-fold (***P = 0.003). Cell extracts were fractionated by 4% to 20% SDS-PAGE gradient gel in reducing conditions. (a) Representative immunoblots. (b) Band density, normalized to the β-actin loading control, was quantitated. (c) ARPE-19 cells were treated with 20 μg/mL of anti-EMP2 diabody for 24 hours. The level of VEGF expression was quantified by Western blot. Anti-EMP2 diabody reduced total VEGF expression by 70% (*P = 0.01). (d) EMP2 levels were decreased by transiently transfecting ARPE-19 cells with 150 pmol EMP2 siRNA and analyzed after 48 hours. As a negative control, the cells were transfected with 150 pmol of scramble control siRNA. The EMP2 siRNA and control siRNA are a pool of four siRNAs targeting EMP2 or a pool of four nontargeting siRNAs, respectively. The level of VEGF expression was quantified by Western blot. EMP2 siRNA reduced total VEGF expression by 57% (**P = 0.005) as compared with control siRNA.