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. Author manuscript; available in PMC: 2014 Apr 1.
Published in final edited form as: J Immunol. 2013 Feb 27;190(7):3049–3053. doi: 10.4049/jimmunol.1203032

Fig. 1. IL-6 signals are required for development of early Bcl6+CXCR5+ Tfh cells.

Fig. 1

Naïve SM (CD45.1+) CD4 T cells were transferred into B6 and IL-6−/− mice and analyzed at day 2 and 3 after LCMV infection. (A) Overlaid Bcl6 histograms of SM cells in infected mice (colors). Naïve CD4 T cells of uninfected mice (gray). (B) IL-2RαintCXCR5hi cells gated and calculated as % of total SM cells. (C) Bcl6+CXCR5+ Tfh cells at day 3 after infection. Percent of Tfh cells calculated. (D) Overlaid IL-2Rα histograms of SM cells in infected mice (colors). Naïve CD4 T cells of an uninfected mouse (gray). IL-2Rα geoMFIs calculated. (E) IL-2Rα geoMFIs of day 3 CXCR5− SM cells. Data are representative of three independent experiments (n = 4–5 mice per group). ** P < 0.01, *** P < 0.001.