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. Author manuscript; available in PMC: 2014 Apr 15.
Published in final edited form as: J Immunol. 2013 Mar 13;190(8):4014–4026. doi: 10.4049/jimmunol.1202963

Figure 4. PSGL1hiLy6Clo compartment consists of fully differentiated Tfh and Th1 cells.

Figure 4

(A–D) LCMV-specific Blimp1-YFP SM (CD45.1+) CD4 T cells were divided into three compartments (PSGL1hiLy6Chi, PSGL1hiLy6Clo, and PSGL1loLy6Clo) in B6 (CD45.2+) mice at day 8 after infection. The three populations were further separated into Blimp1YFP+CXCR5− Th1 and Blimp1YFP−CXCR5+ Tfh cells. (A) Representative FACS plots of SM cells. Gates indicate PSGL1hiLy6Chi, PSGL1hiLy6Clo, and PSGL1loLy6Clo SM populations (left). Blimp1YFP and CXCR5 expression was analyzed (right). (B) Percentages of Blimp1YFP+CXCR5− non-Tfh and Blmp1YFP−CXCR5+ Tfh cells from PSGL1hiLy6Chi, PSGL1hiLy6Clo, and PSGL1loLy6Clo gates calculated. Bcl6 MFIs (C) and Blimp1YFP geometric MFIs (D) calculated. (E–F) Blimp1-YFP B6 mice were infected with LCMV and activated (CD44hiCD62L−) CD4 T cells were analyzed for PSGL1 and Ly6C expression. (E) Blimp1YFP and CXCR5 expression was further examined. (F) Relative frequencies of Blimp1YFP+CXCR5− Th1 and Blimp1YFP−CXCR5+ Tfh cells. N = 3–4 mice per group. * P < 0.05, ** P < 0.01, *** P < 0.001. NS, not statistically different. Shown as mean and SEM.