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. Author manuscript; available in PMC: 2014 Apr 15.
Published in final edited form as: J Immunol. 2013 Mar 13;190(8):4014–4026. doi: 10.4049/jimmunol.1202963

Figure 7. Tfh cells regain IL-7Rα expressions at the peak of the immune response and maintain higher expression of IL-7Rα in memory phase.

Figure 7

(A) Overlaid histograms for IL-7Rα expression by CD44lo CD4 T cells in a naïve mouse (gray filled), and Th1 (blue) and Tfh (red) cells in Th1 and Tfh recipient mice, respectively from day 45 after LCMV infected B6 mice. Quantitation calculated as MFIs. Data are representative of two experiments that were singly done at two different memory time points (day 30 and day 45) after LCMV infection. (B) Naïve SM (CD45.1+) CD4 T cells were transferred into B6 mice that were subsequently infected with LCMV. Th1 (IL-2Rα+CXCR5−) and Tfh (IL-2Rα−CXCR5+) cells were obtained from 3 day LCMV infected spleens. IL-7Rα expression depicted by overlaid histograms (CD44lo CD4 T cells in a naïve mouse, shown in gray filled; Th1 (blue) and Tfh (red) of 3 days LCMV infected mice). (C–F) Naive SM (CD45.1+) CD4 T cells were transferred into B6 mice that were subsequently infected with LCMV. SM cells were analyzed at day 8 (C–D) and 11 (E–F) after LCMV infection. (C) Representative FACS plots of SM cells 8 days after LCMV infection. (D) Overlaid histograms for IL-7Rα expression by CD44lo CD4 T cells in a naïve mouse (gray filled), and Th1 (blue) and Tfh (red) cells from LCMV infected mice at day 8. The bar indicates IL-7Rαhi expressing cells. Quantitation done as IL-7Rαhi % of Th1 (CXCR5−) and Tfh (CXCR5+) cells. (E) SM cells were gated by CD45.1 expression and were subsequently analyzed by IL-7Rα and CXCR5 expression. Numbers in each quadrant indicate frequencies of populations among total SM cells. (F) Overlaid histograms for IL-7Rα expression by CD44lo naïve CD4 (gray filled) of naïve mouse, and Th1 (blue) and Tfh (red) cells from 11 days LCMV infected spleens. The bar indicates IL-7Rα expressing cells. Quantifications made as percentages of IL-7Rα expressing cells within Th1 vs. Tfh cells (middle) and total IL-7Rα MFIs (right). Data are representative of two independent experiments (B–F). n = 4–6 mice per group. * P < 0.05, ** P < 0.01, *** P < 0.001. Shown as mean and SEM.