Skip to main content
. Author manuscript; available in PMC: 2013 Sep 5.
Published in final edited form as: Cell Metab. 2013 Mar 5;17(3):423–435. doi: 10.1016/j.cmet.2013.01.016

Figure 2. Transgenic TLE3 expression alters brown adipose tissue phenotype and function.

Figure 2

A. Immunoblot analysis of TLE3 and UCP-1 protein expression in epididymal WAT and subscapular BAT from control and aP2-TLE3 Tg mice. B. Gross and histological analysis (H&E staining) of subscapular BAT from control and aP2-TLE3 Tg mice. C. Fatty acid uptake and oxidation in primary brown adipose tissue from control and aP2-TLE3 Tg mice was determined as described in Experimental Procedures. D. Oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) from vector or TLE3-expressing brown adipocytes were determined with the Seahorse extracellular flux analyzer. *P < 0.05 by Student’s t-test. E. Energy expenditure in control and aP2-TLE3 Tg mice (N=5/group) determined by indirect calorimetry with Oxymax metabolic cages. F. Time course of core body temperature of control (WT) and aP2-TLE3 Tg mice (N=5/group) housed at 4 °C. Data are presented as mean +/− SEM. Statistical significance was analyzed by repeated measures two-way ANOVA.