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. Author manuscript; available in PMC: 2013 Sep 5.
Published in final edited form as: Cell Metab. 2013 Mar 5;17(3):423–435. doi: 10.1016/j.cmet.2013.01.016

Figure 6. TLE3 and Prdm16 reciprocally regulate the expression of BAT and WAT-selective gene expression.

Figure 6

A. Immunoblot analysis of TLE3 and Prdm16 expression in virally transduced 10T1/2-CAR cells. Stable pools of vector or Prdm16-expressing cells were differentiated with BAT-induction cocktail for 9 days and then transduced with control or TLE3 adenoviral vectors for 3 days. Lysates were collected on Day 12. B. Realtime PCR analysis of BAT- and WAT-selective gene expression in virally-transduced 10T1/2-CAR cells on Day 3 and Day 12 after induction with BAT differentiation cocktail. RNA isolated 3-days after transduction with control or TLE3 adenovirus. C. Realtime PCR analysis of cold-inducible genes in virally-transduced 10T1/2-CAR cells on Day 10 after induction with BAT differentiation cocktail. Cells were also treated with vehicle or 5 μM isoproterenol for 4 h. RNA was isolated 3-days after adenoviral infection.