Table 1.
Location of the mutation |
Viabilityb, % | GTPase activityc, % |
Polymer morphologyd |
Ref. |
|
---|---|---|---|---|---|
Lateral sidea | aa, nº | ZipA | |||
|
WT |
100 |
100 |
B |
This study |
Left |
E83Q |
10 |
40 |
B |
This study |
Left |
R85Q |
0.5 |
5 |
S |
This study |
Left |
D86K |
N |
49 |
Nd |
[11] |
Right | E250A | N | 67 | Nd | [11] |
aThe mutations are positioned on the surface and their location on the structure are shown in Figure 1. Left: the left lateral surface; Right: the right lateral surface.
aa, nº = amino acid number.
bThe % viability was determined from data of Figure 2. N = no growth of ftsZ84 at the restrictive temperature.
cGTPase activity was measured with 12 μM of protein in all cases.
dB, bundles; S, short filaments; Nd, not determined. The shape of the polymers was determined by electron microscopy (Figure 5).