Table 1.

Summary of FtsZ mutations and their effects on cell viability, GTPase activity and polymer morphology

Location of the mutation		Viabilityb, %	GTPase activityc, %	Polymer morphologyd	Ref.
Lateral sidea	aa, nº			ZipA
	WT	100	100	B	This study
Left	E83Q	10	40	B	This study
Left	R85Q	0.5	5	S	This study
Left	D86K	N	49	Nd	[11]
Right	E250A	N	67	Nd	[11]

^aThe mutations are positioned on the surface and their location on the structure are shown in Figure  1. Left: the left lateral surface; Right: the right lateral surface.

aa, nº = amino acid number.

^bThe % viability was determined from data of Figure  2. N = no growth of ftsZ84 at the restrictive temperature.

^cGTPase activity was measured with 12 μM of protein in all cases.

^dB, bundles; S, short filaments; Nd, not determined. The shape of the polymers was determined by electron microscopy (Figure  5).