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. 2013 Apr 15;8(4):e61440. doi: 10.1371/journal.pone.0061440

Figure 4. Effect of viral PLA2 on the morphology of UT/7-Epo cells. (A) Release of free fatty acid from UT7-Epo cells.

Figure 4

Cells were treated for 2 h or 4 h with the different mutated VP1u proteins. Blank: RPMI-1640 medium; Mock: UT7-Epo cells without protein treatment; MBP, VP1u, VP1u(H153A), or VP1u(D195A): UT7-Epo cells were treated with purified MBP, VP1u, VP1u(H153A) or VP1u(D195A) proteins. Data are representative of three independent experiments. (B) Cell morphological observation after treatment with various proteins. Mock: normal UT7-Epo cells without protein treatment; MBP, VP1u(WT), VP1u(H153A), and VP1u(D195A): cells were treated with MBP, VP1u(WT), VP1u(H153A) and VP1u(D195A) proteins, respectively, for 12 h, 24 h or 48 h. Images were taken at a magnification of ×20 (objective lens).