Figure 6. Transcriptional activation effects of mutant VP1u on NF-κB, GAS, CRE and TNF-α signal pathways.

HEK293T cells were co-transfected with reporter plasmid DNA and the indicated WT- or mutant VP1u-expressing plasmids. pcDNA3.1 vector was used to adjust the transfected DNA in a total amount of 200 ng. Luciferase activity was determined at 48 h post-transfection. Data are representative of three independent experiments. An asterisk marker indicates a significant difference in the luciferase activity between the control and tested groups.