Figure 2. Effect of NEM and IAA concentrations on endogenous nuclear caspase-2 levels.

(A) Cells were pretreated with the indicated concentrations of NEM for 10 min before lysis. (B) Effect of NEM concentrations on nuclear caspase-2 levels. Cells were pre-treated with NEM as indicated for 1 h before lysis. (C) Effect of 10 min or 1 h pre-treatment with the indicated concentrations of Iodoacetic Acid (IAA) or NEM on caspase-2 levels in the nuclear fraction.