Table 1.
Nested-PCR primers
| |
|
CSNK2B |
LY6G5B |
||
|---|---|---|---|---|---|
| First Round | Second Round | First Round | Second Round | ||
|
H.sapiens |
F |
HsCSNK2BE1f01 |
Hs-MamCSNK2BE1-2f01 |
HsLY6G5BE1f01 |
HsLY6G5BE1f02 |
| CGTTCCCTGGAAGTAGCAA |
GCTGACGTGAAGATGAGCAG |
GAGCATGGTCACAGGAAGGT |
CATCTCCCCAGAATTCCAAA |
||
|
R |
Hs-SsCSNK2BE7r02 |
Hs-Mam-RnCSNK2BE7r01 |
HsLY6G5BE3r02 |
Hs-MamLY6G5BE3r01 |
|
| CCCACCACAATAACGACTCC |
TCAGCGAATCGTCTTGACTG |
CGGAGGCCTAAGAAATCACA |
TGTTTTCAGAGAGGGCAGTG |
||
|
M.mulata |
F |
MamCsnk2bE1iso1f01 |
Hs-MamCsnk2bE1-2f01 |
MamLy6g5bE1f01 |
MamLy6g5bE1f02 |
| ACCCTCCCCAATTTCCACT |
GCTGACGTGAAGATGAGCAG |
TCACAGGAAGGTGGGGTTT |
CGTCTCCCCAGAATTCCATA |
||
|
R |
Mam-BtCsnk2bE7r02 |
Hs-Mam-RnCsnk2bE7r01 |
MamLy6g5bE3r02 |
Hs-MamLy6g5bE3r01 |
|
| TCCCACCACAATAATGACTCC |
TCAGCGAATCGTCTTGACTG |
CAACAGAGGGAGGCCTAAGA |
TGTTTTCAGAGAGGGCAGTG |
||
|
S.scrofa |
F |
SsCsnk2bE1f01 |
SsCsnk2bE1-2f01 |
SsLy6g5bE1f01 |
SsLy6g5bE1f02 |
| TCCTTGGAAGCAGAAACTCC |
CGCTGAAGTGAAGATGAGCA |
CTCAGGGATCACCCCTCTC |
TCACGTGCTCGTAGGTATGC |
||
|
R |
Hs-SsCsnk2bE7r02 |
SsCsnk2bE7r01 |
SsLy6g5bE3r02 |
SsLy6g5bE3r01 |
|
| CCCACCACAATAACGACTCC |
GGGAATCAGCGAATTGTCTT |
GAATGCTGGGGTCTTAGGG |
CCGGGAAGGATGAGATGTTA |
||
|
B.taurus |
F |
BtCsnk2bE1f01 |
BtCsnk2bE1-2f01 |
BtLy6g5bE1f01 |
BtLy6g5bE1f02 |
| GAAGCAGAAACTCCCCTTCC |
CCGACGTGAAGATGAGCAG |
GTCAGAACCACCCTGCAGTT |
CTCTCCCCAGAAGTCCATGA |
||
|
R |
Mam-BtCsnk2bE7r02 |
BtCsnk2bE7r01 |
BtLy6g5bE3r02 |
BtLy6g5bE3r01 |
|
| TCCCACCACAATAATGACTCC |
GGAATCAGCGAATCGTCTTC |
GGGGTGTCAGGAGTGAGATG |
AATCTATCTGCGACGGGAAA |
||
|
R.norvegicus |
F |
Rn-MumCsnk2bE1f01 |
Rn-MumCsnk2bE1-2f01 |
RnLy6g5bBE1f01 |
RnLy6g5bE1f02 |
| GTTCCTTGGAAGCACAGCTC |
CCGCGGACATAAAGATGAGT |
AGTGTGATCCCAGGAAGGTG |
CTACTCCACGGGAGTTGCTC |
||
|
R |
RnCsnk2bE7r02 |
Hs-Mam-RnCsnk2bE7r01 |
RnLy6g5bE3r02 |
RnLy6g5bE3r01 |
|
| GTGTCACAGGCAGAGGAGGT |
TCAGCGAATCGTCTTGACTG |
CCATGGAGAGCAGAAGGAAG |
CAGAGCAGAATCTGGGAAGG |
||
| M.musculus |
F |
Rn-MumCsnk2bE1f01 |
Rn-MumCsnk2bE1-2f01 |
MumLy6g5bE1f01 |
MumLy6g5bE1f02 |
| GTTCCTTGGAAGCACAGCTC |
CCGCGGACATAAAGATGAGT |
ACTGCCTGTCAAACCCATTC |
TCCCCCAAATTCCATAATGA |
||
| R | MumCsnk2bE7r02 |
MumCsnk2bE7r01 |
MumLy6g5bE3r02 |
MumLy6g5bE3r01 |
|
| AGCAGAGGAATGGTGGTGTC | GTGGGCAATCAGCGAATAGT | GAGTGTTCACAGACCGCAGA | GGGGAACACATCAGGGTCTA | ||
Name and sequence (5’-3’ orientation). F, forward primer; R, reverse primer.