Table 2.

Expression constructs primers

Long primers for V5 epitope cloning in pcDNA3.1/Zeo(-)
Name	Sequence
MCV5_F01	GGGGCGGCCGCATGGGAAAGCCGATCCCAAACCCTCTATTAGGTCTGGACTCCACCGGATCCTGAGATATCGGG
MCV5_R01	CCCGATATCTCAGGATCCGGTGGAGTCCAGACCTAATAGAGGGTTTGGGATCGGCTTTCCCATGCGGCCGCCCC
Primers for CSNK2B, LY6G5B and CSNK2B-LY6G5B Chimera ORFs cloning in pcDNA3.1/V5-His-TOPO
Name	Sequence
HsCSNK2BORF_F01	CCCGGATCCATGAGCAGCTCAGAGG
HsCSNK2BORF_R01	CCCACCGGTGCGAATCGTCTTGAC
HsLY6G5BORF_F01	CCCGGATCCATGAAGGTCCATATGC
HsLY6G5BORF_R01	CCCACCGGTGGAAGGGTGAGGTGTC
Primers for final construct in pcDNA3.1/Zeo(-)
Name	Sequence
HsCSNK2BORF_F01	CCCGGATCCATGAGCAGCTCAGAGG
HsLY6G5BORFE2_F02	CCCGGATCCGTTCCTGTTCCCGACATC
Hisx6HindIIIR_01	CCCAAGCTTTCAATGGTGATGGTGATGATG
HsLY6G5BPS_F01	GGGTCTAGAATGAAGGTCCATATGC
HsLY6G5BPS_R01	CCCGCGGCCGCTCTTTCCTACTGTGAA

Restriction enzyme target sequences are in bold and italics.