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. 2013 Apr 15;8(4):e61305. doi: 10.1371/journal.pone.0061305

Figure 3. Effects and duration of transgene expression in baculovirus-infected hUCB-MSCs.

Figure 3

A: Cell viability of hUCB-MSCs infected with Bac-NIS at different MOIs at 24 hpi as detected by the CCK-8 assay. B: Cell proliferation of hUCB-MSCs infected by Bac-NIS at different MOIs over time (1, 3, 5, 7, 9, 11, 13 and 15 dpi), as detected by the CCK-8 assay. The cells were seeded 1 day before infection with Bac-NIS and reseeded at 8 dpi when the cells reached ∼80% confluency. C: GFP+ % of Bac-GFP-infected hUCB-MSCs over time (1, 2, 3, 4, 8 and 12 dpi). The cells were reseeded at 8 dpi when reached ∼80% confluency. Results are means ± SD (n = 3). Abbreviations: Bac-NIS, recombinant baculovirus carrying sodium-iodide symporter reporter gene; dpi, days post-infection; CCK-8, Cell Counting Kit-8.