Skip to main content

View full-text article in PMC

. 2013 Apr 5;14:43. doi: 10.1186/1471-2350-14-43

Table 1.

Primers and PCR conditions

Primer	bp	Primer sequence 5^′to 3^′	PCR conditions
Hprt1F	94	TGACACTGGCAAAACAATGCA	95°C 10^′ (95°C 40″; 60°C 30″; 72°C 30″) × 40
Hprt1R	94	GGTCCTTTTCACCAGCAAGCT	Dissociation curve
Rn18SF	178	CTCTGGTTGCTCTGTGCAGT	95°C 10^′ (95°C 40″; 62°C 30″; 72°C 30″) × 40
Rn18SR	178	GGCTCCTTGTAGGGGTTCTC	Dissociation curve
Mrps28SF	223	GAAATGCAAGCACGGAGAGT	95°C 10^′ (95°C 40″; 62°C 30″; 72°C 30″) × 40
Mrps28SR	223	CCGATGACCAGTTTGTCCTT	Dissociation curve
VegfaF	200	GCTACTGCCGTCCGATTGAGAC	95°C 10^′ (95°C 40″; 65°C 30″; 72°C 30″) × 40
VegfaR	200	GTGCTGGCTTTGGTGAGGTTTG	Dissociation curve
Hif1aF	209	GCTTCTGTTATGAGGCTCACC	95°C 10^′ (95°C 40″; 62°C 30″; 72°C 30″) × 40
Hif1aR		TCAAACTGAGTTAACCCCATGT	Dissociation curve