Figure 5. (A) HCT116, SW480 and HCT116 p53−/− cells were treated with different concentrations of ellipticine or NSC176327 for 12 h and fold induction of p53 transcriptional response was measured by bioluminescence imaging of human p53 reporter, PG-13-luc. (B) HCT116, SW620 and HCT116 p53−/− cells were treated with different concentrations of ellipticine or NSC176327 for 16 h and protein expression level of p53, p21 and DR5 was determined by western blot. Ran was included as loading control.