Figure 6. (A) HCT116, SW620 and HCT116 p53−/− cells were treated with different concentrations of ellipticine or NSC176327 for 12 h and protein expression level of p73 was determined by western blot. Ran was included as loading control. (B) HCT116 p53−/− cells or HCT116 p53−/− cells transduced with retroviral vector containing TAp73 RNAi were treated with 5 μM or 10 μM NSC176327 or 20 μM ellipticine for 24, 48 or 72 h and cell viability was measured by MTT assay.