Table 2.
Primer sets used for AASS mutation analysis
| Amplicon | Sequence (5′>3′)1 | Exons | Regions of cDNA |
|---|---|---|---|
| A |
[-21M13]-cgattggcagatgagaaggt |
1 |
(c.–221-c.–16) |
| [M13-Rev]-atctccaccgcatctcacag | |||
| B |
[-21M13]-cacttgacatcccagttttcc |
2 |
(c.–15-c.210) |
| [M13-Rev]-ttcctcagctggagtaagca | |||
| C |
[-21M13]-tgttgtgcctttgctacaca |
3 |
(c.211-c.387) |
| [M13-Rev]-tcccatctgaaaaacaaggtag | |||
| D |
[-21M13]-ttgctacctggcgttttctaa |
4 |
(c.388-c.472) |
| [M13-Rev]-cttgccgcagaaaagagaaa | |||
| E |
[-21M13]-catgcagattggagaacgag |
5 & 6 |
(c.473-c.687) |
| [M13-Rev]-atggctgcccacatcatt | |||
| F |
[-21M13]-ggaaggcaagtggagctatg |
7 & 8 |
(c.688-c.894) |
| [M13-Rev]-tgggcacatgtagacctgaa | |||
| G |
[-21M13]-tttcttcggcatgcaataca |
9 |
(c.895-c.1043) |
| [M13-Rev]-ctgccaagaggtcaagaaaga | |||
| H |
[-21M13]-gcagagtcctgaagaatgagc |
10 & 11 |
(c.1044-c.1278) |
| [M13-Rev]-ccccaagagacaagtaagcag | |||
| Internal rev seq primer cagcaacccatctcacat | |||
| I |
[-21M13]-gggcagagttgattgcttgt |
12 & 13 |
(c.1279-c.1406) |
| [M13-Rev]-gccagccacttagtttggat | |||
| J |
[-21M13]-ttgtggaatgcaagattctg |
14 & 15 |
(c.1407-c.1655) |
| [M13-Rev]-tgatttgtgcaccttctgga | |||
| Internal rev seq primer cagaaacaaagtagtcttc | |||
| K |
[-21M13]-gagtgcctgtgtctttttgg |
16 & 17 |
(c.1656-c.1875) |
| [M13-Rev]-gaacctgggagatggaggtt | |||
| Internal forw seq primer ctgagtggatccatggcattg | |||
| L |
[-21M13]-tcaaatggtacatgctttgaaga |
18 |
(c.1876-c.2016) |
| [M13-Rev]-gggtttgggatcagggagta | |||
| M |
[-21M13]-ttctgttgctttctttgttcg |
19 |
(c.2017-c.2184) |
| [M13-Rev]-caatcaatcataagattcctgaaaaa | |||
| N |
[-21M13]-gacaggaaaacctgctaggc |
20 |
(c.2185-c.2280) |
| [M13-Rev]-gactcccatcactgggtcac | |||
| O |
[-21M13]-ttgaggtgtatttgaagttcagtg |
21 & 22 |
(c.2281-c.2485) |
| [M13-Rev]-acatcttcccattcctgctg | |||
| Internal rev seq primer ctacccacattagagcaacg | |||
| P |
[-21M13]-ggcaggaggagatgacagac |
23 |
(c.2486-c.2662) |
| [M13-Rev]-actcagccaccttggaactg | |||
| Q |
[-21M13]-aaaatgcctaggcctccaag |
24 |
(c.2663-c.*187) |
| [M13-Rev]-gtggcttgcatctcctgttc | |||
| R |
5′-tctccaagggtcttcaccac-3′ |
|
c.47-c.66 |
| 5′-agaatgccaccagctttgac-3′ |
|
c.236-c.217 |
|
| S |
5′-agttcctcaggcagagtcca-3′ |
|
c.2421-c.2440 |
| 5′-ggctgaaaagccattgatgt-3′ |
|
c.2607-c.2588 |
|
| T |
5′-gccttccatcccagtttctt-3′ |
|
c.–116-c.–97 |
| 5′-ctgcatctctcaccacagga-3′ |
|
c.1323-c.1342 |
|
| Internal seq. primer 5′-tgcatttttctcccacacaa-3′ |
|
c.318-c.337 |
|
| Internal seq. primer 5′-cccaaactggagacctcaga-3′ |
|
c.755-c.774 |
|
| U |
5′-gaatgctttggagacatgctt-3′ |
|
c.1237-c.1257 |
| 5′-ggtgtattgcctgggaagaa-3′ |
|
c.*23-c.*42 |
|
| Internal seq. primer 5′-ctgcaagctacatcacaccag-3′ |
|
c.1724-c.1744 |
|
| Internal seq. primer 5′-tggcatttcttctgctcaca-3′ | c.2136-c.2155 |
1All forward and reverse primers were tagged with a –21M13 (5′-TGTAAAACGACGGCCAGT-3′) sequence or M13rev (5′-CAGGAAACAGCTATGACC-3′) sequence.