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. 2013 Apr 15;8(4):e61940. doi: 10.1371/journal.pone.0061940

Figure 4. Proteins encoded by cdCSP1 and cdCSP2 are specifically expressed and located in chlamydospores.

Figure 4

C. dubliniensis wild type Wü284 (A) and the C. dubliniensis GFP reporter strains Cd30750G1A/B (cdCsp1-GFP) (B) and Cd40770G1A/B (cdCsp2-GFP) (C) were grown in YPD and Staib liquid medium for 28 h at 25°C, and on rice-extract agar for 3 d at 25°C, respectively, and inspected by phase contrast and fluorescence microscopy. Fluorescence microscopy demonstrated that the genes of interest are specifically induced during growth in Staib medium and that the encoded proteins exclusively localize to chlamydospores. The two independently constructed A/B-GFP reporter strains behaved identically and only one of them is shown (scale bar: 10 µm).