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. Author manuscript; available in PMC: 2013 Nov 1.
Published in final edited form as: J Invest Dermatol. 2013 Jan 10;133(5):1302–1310. doi: 10.1038/jid.2012.479

Figure 5. TGF-β induces p300-mediated histone H4 hyperacetylation.

Figure 5

a. Fibroblasts were incubated with TGF-β2 (12.5 ng/ml) for 24 h. Total histones were extracted and analyzed by Western blotting followed by densitometry. Lower panel, quantitation. The results show levels of AcH4 histone normalized to total H4 histone (mean ± S.D) from three independent experiments(lower panel). *p<0.05. b. Rat-12 fibroblasts stably expressing conditional p300 were infected with Ad-β-gal or Ad-Tet to induce p300 expression, and whole cell lysates were examined by Western analysis. Arrow indicates actin bands. c. Foreskin fibroblasts transfected with p300-specific ribozyme or inactive ribozyme were incubated with TGF-β2 (12.5 ng/ml) for 48 h. Whole cell lysates were analyzed by Western blot.