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. Author manuscript; available in PMC: 2014 May 1.
Published in final edited form as: Eur J Pharm Biopharm. 2012 Dec 27;84(1):49–62. doi: 10.1016/j.ejpb.2012.12.012

Figure 9.

Figure 9

The effect of DOX encapsulation in PLA-V6K2 NPs on DNA damage and apoptosis of MTCL (a) and 4T1 (b) mouse breast cancer cells; Groups included PBS, PLA-V6K2 NPs, free DOX (1 μM), and DOX in PLA-V6K2 NPs (1 μM); tumor cells were incubated with drug-loaded NPs for 2 h; Next, the medium was replaced with drug-free medium and incubated for zero, 4, and 22 h. The response was determined by the expression of γ-H2AX DNA damage marker, cleaved PARP apoptosis marker, and Cyclin B1 cell cycle arrest marker); quantified intensities of γ-H2AX (c) and cleaved PARP (d) markers; GAPDH was used as the internal control and the amount of the markers in PBS-treated cells at time zero was set as one.