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Structural and functional analysis of the 5′-regionAgo2. The folding potential (A) and predicted 2D structure of the 5′-region of mRNAAgo2 (B) suggest two GC-helices. A luciferase gene containing the mRNAAgo2 5′-region (pRL-Ago2) but not a derivative mutated in the GC-helix region nor the wt gene (C and D) indicate Ago2-mediated up-regulation of gene expression (NHA-Ago2).
