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. 2013 Apr 8;13:183. doi: 10.1186/1471-2407-13-183

Figure 2.

Figure 2

Detection of EGFR 3′UTR polyA tract somatic mutations, using fluorescent multiplex PCR. The profile generated from malignant tissue (red) was superimposed on that obtained from distant non-malignant tissue (blue) after alignment of the control amplicons (peaks to the right corresponding to PCBD2). A: Pattern observed in a non mutated sample with A13/A14 genotype. B: Pattern observed in a mutated sample with A13/A14 genotype; notice in the tumor sample a shift of the peaks to the left corresponding to A11 and A12 repeats.