Figure 1.

Distinct growth patterns of lung cancer cell lines in rBM 3-D organotypic culture. Four human and mouse lung cancer cell lines were cultured in rBM 3-D culture. Morphogenesis of lung cancer cells was visualized by filamentous actin staining and confocal fluorescent microscopy (insets). The insets of A-C were captured at 400x and part D was captured at 200x because of the larger diameter of LLC cell cluster. The growth patterns of lung cancer cells in vivo were visualized using H & E staining (main images, 100x). Representative glandular structures are indicated by arrows in A & C.