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. 2013 Feb 14;13:16. doi: 10.1186/1475-2867-13-16

Figure 3.

Figure 3

Activated expression of the tumor promoting genes by TGF-β1 in rBM 3-D. Total cell RNA was extracted from A549 cells cultured in rBM 3-D (Mat) culture exposed to the indicated combinations of TGF-β1 and Col-1. The expression of the selected tumor-promoting genes was determined using quantitative RT-PCR. Ratios of each mRNA over the housekeeping gene 36B4 were compared across various culture conditions. A-C) The expression of Myc, PAI-1, and LOX was evaluated, respectively. A fold change was obtained by setting the values from the control group to one. D) The expression of Myc, PAI-1, and LOX was compared between A549 cells cultured in Mat+TGF-β1+Col-1 with or without PP2. A fold change was obtained by setting the values from the PP2 minus group to one. E) Similar to part C except that the expression of Myc, PAI-1, and LOX was compared between A549 cells cultured in Mat+TGF-β1 with or without PP2. Mean and standard deviations were obtained from three independent experiments. *, **, and *** indicate a P value < 0.05, 0.01, and 0.001, respectively.