Table 3.
Comparisons | qRT-PCR | GME | m a x ( P P L R ,1− P P L R ) | Consistency | |
---|---|---|---|---|---|
11q13+ vs. 11q13- |
ORAOV1-201 |
+ |
+ |
1.0000 |
Y |
|
ORAOV1-202 |
+ |
+ |
0.9968 |
Y |
|
NEO1-201 |
+ |
+ |
0.8961 |
Y |
|
NEO1-202 |
+ |
+ |
0.9719 |
Y |
ORAOV1-201 vs. 202 |
11q13+ |
+ |
+ |
0.9154 |
Y |
|
11q13- |
+ |
+ |
0.5782 |
Y |
NEO1-201 vs. 202 |
11q13+ |
- |
- |
0.9999 |
Y |
11q13- | - | - | 1.0000 | Y |
The expression changes between groups 11q13+ and 11q13- for each transcript, and between two transcripts of the same gene for each group, are examined. The results of qRT-PCR and GME are “+” or “-” for up- and down-regulation in the first comparison component, respectively. Column of max(PPLR,1-PPLR) gives the probability of differential expression. The concordances between qRT-PCR validation and GME results are given in the right-most column.