Table 2.
Primer sequences used for multiplex PCR amplification panels
| Gene |
SNP (rs) |
Forward (5’→3’) |
Reverse (5’→3’) |
Amplicon size (bp) | PCR mix (μM) |
|---|---|---|---|---|---|
|
Panel 1 | |||||
|
IL1-α |
1800587 |
GCAATAGACCTTATGACACCTAAC |
GGTAGAGAAGAGAACAGTGGTATT |
731 |
3.0 |
|
IL1-β |
16944 |
AGTATATGTGGGACAAAGTGGAAG |
CTCATCTGGCATTGATCTGGTT |
389 |
1.4 |
|
IL6 |
1800795 |
AGCCTGTTAATCTGGTCACTGA |
CTTGTGGAGAAGGAGTTCATAGC |
498 |
3.2 |
|
IL10 |
1800872 |
TTCTATGTGCTGGAGATGGTGTA |
GGGTGGGCTAAATATCCTCAAAG |
385 |
1.4 |
|
IL12 |
11575934 |
GGTTAAGTGACTGGTGCCAAG |
TCCTGTACTCAGAGTGATCTTACC |
210 |
3.0 |
|
Panel 2 | |||||
|
TLR2 |
5743708 |
TGCTGCCATTCTCATTCTTCCTG |
TCCTCAAATGACGGTACATCCA |
282 |
1.4 |
|
IL10 |
1800896 |
ATCTGAAGAAGTCCTGATGTCACT |
ACCATCTCCAGCACATAGAATGA |
367 |
1.4 |
|
TLR2 |
4696480 |
CATAGTTGTCACAGTCCCTTGG |
TGGCATGATCTCGGCTCAC |
346 |
4.0 |
|
IL6 |
1800797 |
GGTGAAGAGACTCAGTGGCAAT |
AGTGACCAGATTAACAGGCTAGAAT |
444 |
2.0 |
|
TLR4 |
4986791 |
CCGATTAGCATACTTAGACTACTACCT |
AGACTGGACAAGCCATTGAAGAT |
555 |
3.2 |
|
Panel 3 | |||||
|
IL12 |
401502 |
ACCTCTGTATGACATTGAGTAAGC |
GAACCAACGGGACCACCAT |
285 |
1.0 |
|
TLR2 |
121917864 |
CTGTGCTCTGTTCCTGCTGAT |
GAAATGGGAGAAGTCCAGTTCATAC |
382 |
1.0 |
|
TLR9 |
187084 |
ATTCATTCATTCAGCCTTCACTCAG |
CCATCCAGCCTTCTTACAAACCT |
324 |
1.0 |
|
CD14 |
2569190 |
GGCTTCCAGGCTTCACACT |
AGGACACTGCCAGGAGACA |
236 |
1.0 |
|
TLR4 |
4986790 |
TCCCTGAACCCTATGAACTTTATCC |
AGGCTTGGTAGATCAACTTCTGAA |
494 |
1.0 |
| CD14 | 2569191 | AAGGAAGGGATGGGACTATGTT | CAGGAATCTGAGGCAAGAGAAT | 347 | 1.0 |