Table 3.

Extension probes used for multiplex primer extension

Gene	Positionchange	Variation	Extension probe (5’→3’)	Probe size (bp)	PCR mix (μM)
Panel 1
IL1-α	−889	C>T	TTAATAATAGTAACCAGGCAACA	23	2.0
IL1-β	−511	A>G	TTTTTTTTTTGGTGCTGTTCTCTGCCTC	28	1.2
IL6	−174	C>G	TTTTTTTTTTTTTTTCCCCTAGTTGTGTCTTGC	33	2.0
IL10	−592	A>C	TTTTTTTTTTTTTTTTTTTTTCCTGTGACCCCGCCTGT	38	1.2
IL12	+705	A>G	TTTTTTTTTTTTTTTTTTTTTTTTTTCCAGCTCCGACGACGGC	43	2.0
Panel 2
TLR2	+2259	A>G	TTTTTTTGCGCTTCTGCAAGCTGC	24	0.8
IL10	−1082	A>G	TTTTTTTTCACTACTAAGGCTTCTTTGGGA	30	0.8
TLR2	−16933	A>T	TTTTTTTTTTTTTTTTTATTGAAGGGCTGCATCTGG	36	3.2
IL6	−596	A>G	TTTTTTTTTTTTTTTTTTTTTGTAACTGCACGAAATTTGAGG	42	0.8
TLR4	+1196	C>T	TTTTTTTTTTTTTTTTTTTTTTTTGTTCTCAAAGTGATTTTGGGACAA	48	3.2
Panel 3
IL12	+1196	C>G	TTTTCAGGTGGCAAGGCCCC	20	1.0
TLR2	+2029	C>T	TTTTTCCCTTCAAGTTGTGTCTTCATAAGC	30	1.4
TLR9	−1486	C>T	TTTTTTTTTTTTTTCAGATAAAAGATCACTGCCCT	35	1.0
CD14	−260	A>G	TTTTTTTTTTTTTTTTTTTTGGATGTTTCAGGGAGGGGGG	40	1.0
TLR4	+896	A>G	TTTTTTTTTTTTTTTTTTTAGCATACTTAGACTACTACCTCGATG	45	1.0
CD14	−159	C>T	TTTTTTTTTTTTTTTTTTTTTTTGAGGATTAATTAGTAACTCACCAGTTT	50	2.0