Table 4.
List of bacterial strains and plasmids used in this study
| Strain or plasmid | Descriptiona | Source or reference |
|---|---|---|
| Strains | ||
| E. coli DH5α | supE44 Δ(lacZYA-argF) U169 (Φ80lacZ ΔM15) hsdR17 recA endA1 gyrA96 thi-1 relA1 | Invitrogen |
| E. coli BW25113 | lacIqrrnBT14ΔlacZWJ16hsdR514 ΔaraBADAH33ΔrhaBADLD78 | 42 |
| E. coli BW25113 (ΔgdhAΔaceE, PT5-dxs) | E. coli BW25113, ΔgdhA, ΔaceE, replacement of native promoter of the dxs gene with T5 promoter | 19 |
| E. coli CBW12241 | CIChE strain of E. coli BW25113 (ΔgdhAΔaceE, PT5-dxs) resistance to 8 μM triclosan, ΔrecA | This study |
| E. coli CBW12241 (PT5-appY) | E. coli CBW12241, replacement of native promoter of the appY gene with T5 promoter | This study |
| E. coli CBW12241 (ΔiclR) | E. coli CBW12241, ΔiclR | This study |
| E. coli CBW12241 (ΔiclR, PT5-appY) | E. coli CBW12241(PT5-appY), ΔiclR | This study |
| E. coli CBW12251 | E. coli CBW12241 (ΔiclR, PT5-appY) with an additional chromosomal copy of dxs under the control of T5 promoter | |
| Plasmid | ||
| pP21KF3T5b | CIChE integration expression vector, Kanr | 29 |
| pP21KF3T5b-crtEBIipi | pP21KF3T5b derivative containing crtE, crtB and crtI gene from Pantoea agglomerans and ipiHPI from Haematococcus pluvialis, Kanr | This study |
| pAH121 | Helper plasmid expressing phage P21 Int, Ampr | 28 |
| pBAD24-WZM1 | pBAD24 derivative containing crtE, crtB and crtI gene from Pantoea agglomerans and ipiHPI from Haematococcus pluvialis, Ampr | 19 |
| pSIM6 | pSC101 repliconts PL-gam-bet-exo cI857, Ampr | 43 |
| pKD4 | oriRγ, FRT::kan::FRT template plasmid, Kanr, Ampr | 42 |
| pCP20 | pSC101 repliconts Flp(λRp) cI857, Cmr, Ampr | 42 |
| pBAD24 | pMB1 ori, PBAD L-arabinose inducible, Ampr | 41 |
| pBappY | pBAD24 derivative containing the appY gene from E. coli, Ampr | This study |
| pBidi | pBAD24 derivative containing the idi gene from E. coli, Ampr | This study |
| pBpck | pBAD24 derivative containing the pck gene from E. coli, Ampr | This study |
| pBpps | pBAD24 derivative containing the pps gene from E. coli, Ampr | This study |
| pBrpoS | pBAD24 derivative containing the rpoS gene from E. coli, Ampr | This study |
| pBycgW | pBAD24 derivative containing the ycgW gene from E. coli, Ampr | This study |
| pByjiD | pBAD24 derivative containing the yjiD gene from E. coli, Ampr | This study |
| pQE30 | ColE1 ori, PT5 IPTG inducible, Ampr | Qiagen |
| pQEwrbA | pQE30 derivative containing the wrbA gene from E. coli, Ampr | This study |
| pQydeO | pQE30 derivative containing the ydeO gene from E. coli, Ampr | This study |
| pQatpE | pQE30 derivative containing the atpE gene from E. coli, Ampr | This study |
| pQdxs | pQE30 derivative containing the dxs gene from Bacillus subtilis, Ampr | This study |
aAmpr: ampicillin resistance; Cmr: chloramphenicol resistance; Kanr: kanamycin resistance; crtE: geranylgeranyl diphosphate synthase; crtB: phytoene synthase; crtI: phytoene desaturase; ipiHPI: isopentenyl diphosphate isomerase from Haematococcus pluvialis; appY: DNA-binding transcriptional activator; idi: isopentenyl diphosphate isomerase; pck: phosphoenolpyruvate carboxykinase; pps: phosphoenolpyruvate synthetase; rpoS: RNA polymerase sigma 38; ycgW: inhibitor of σS proteolysis; yjiD: inhibitor of σS proteolysis; wrbA: NAD(P)H: quinone oxidoreductase; ydeO: DNA-binding transcriptional dual regulator; atpE: ATP synthase, F0 complex, c subunit; dxs: 1-deoxy-D-xylulose-5-phosphate synthase.