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. 2013 Feb 7;13:29. doi: 10.1186/1471-2180-13-29

Figure 1.

Figure 1

Denaturing gradient gel electrophoresis (DGGE) fingerprints of bacterial 16S rRNA gene fragments amplified from stem and leaf DNA templates obtained from four genotypes of Lippia sidoides using the primers (a) U968/L1401 [26] and (b) 799F/1492R [29] followed by U968/L1401. Two gels were used to compose this figure. Lanes 1, 2, 3, 4, 1′, 2′, 3′, 4′ – stem samples and 5, 6, 7, 8, 5′, 6′, 7′, 8′ – leaf samples from genotypes LSID003, LSID006, LSID104 and LSID105, respectively. Lanes marked with M correspond to a 1 kb ladder (Promega). Letters A and B followed by numbers indicate bands that were extracted from the gels a and b, respectively, for sequence analysis. The right side shows the corresponding dendrograms obtained after cluster analysis with mathematical averages (UPGMA) and Dice similarity coefficients comparing the total bacterial 16S rRNA gene fragments amplified from stem and leaf DNA templates obtained from four genotypes of L. sidoides. The genotypes are represented by the three first numbers (LSID - 003, 006, 104 and 105), followed by C or F for stem and leaf samples, respectively, and T1 and T2 corresponding to the replicates.