. 2013 Feb 7;13:29. doi: 10.1186/1471-2180-13-29

Table 2.

Universal bacterial primers and group-specific primers (based on 16S rRNA) and fungal primers (based on 18S rRNA) used for PCR amplification of L. sidoides stem and leaf DNA for DGGE evaluation

Communities	Primers	Reference	Sequences ^a
Total bacteria	*U968/L1401	[26]	*5′ACCGCGAAGAACCTTAC3′/
Total bacteria	*U968/L1401	[26]	5′GCGTGTGTACAAGACCC3′
Total bacteria	799F/1492R	[29]	5′AACMGGATTAGATACCCKG3′/
Total bacteria	*U968/L1401	[26]	5′TACGGYTACCTTGTTACGACT3′
Alphaproteobacteria	F203α/L1401	[30]	5′CCGCATACGCCCTACGGGGGAAAGATTTAT3′
Alphaproteobacteria	*U968/L1401	[26]	5′CCGCATACGCCCTACGGGGGAAAGATTTAT3′
Betaproteobacteria	F948β/L1401	[30]	5′CGCACAAGCGGTGGATGA3′
Betaproteobacteria	*U968/L1401	[26]	5′CGCACAAGCGGTGGATGA3′
Actinobacteria	F243/L1401	[27]	5′ GGATGAGCCCGCGGCCTA 3′
Actinobacteria	*U968/L1401	[26]	5′ GGATGAGCCCGCGGCCTA 3′
Fungi	EF4/ITS4	[28]	5′GGAAGGGRTGTATTTATTAG3′/
	*ITS1f/ITS2	[24]	5′ TCCTCCGCTTATTGATATGC3′
	*ITS1f/ITS2	[25]	*5′CTTGGTCATTTAGAGGAAGTAA3′/
		[24]	5′GCTGCGTTCTTCATCGATGC3′

^a The sequences correspond to the primers in bold.

* Primer with a 40 bp GC-clamp (5^′- CGCCCGCCGCGCGCGGCGGGCGGGGCGGGGGCACGGGGGG –3^′) attached.