Table 2.

Sequences of primers used for PCR

Gene and polymorphism	Primer 5′-3′	Annealing temperature [°C]
TLR2 Arg677Trp	Forward: ccc ctt caa gtt gtg gct tca taa g	65
TLR2 Arg677Trp	Reverse: agt cca gtt cat act tgc acc ac
TLR2 Arg753Gln	Forward: cat tcc cca gcg ctt ctg caa gct cc	65
TLR2 Arg753Gln	Reverse:: gga acc tag gac ttt atc gca gct c
TLR4 Asp299Gly	Forward: agc ata ctt aga cta cta cct cca tg	62
TLR4 Asp299Gly	Reverse: aga aga ttt gag ttt caa tgt ggg
TLR4 Thr399Ile	Forward: ggt tgc tgt tct caa agt gat ttt ggg aga a	60
TLR4 Thr399Ile	Reverse: gga aat cca gat gtt cta gtt gtt cta agc c