Table 3.
Restriction enzymes and length of the restriction fragments
| Gene and polymorphism | Restriction enzyme | Restriction temperature [°C] | Fragment length [bp] |
|---|---|---|---|
| TLR2 Arg677Trp |
MwoI |
60°C |
Wild type (allele C): 130 bp + 22 bp |
| (rs unknown) |
Arg677Trp (allele T): 152 bp |
||
| TLR2 Arg753Gln |
MspI |
37°C |
Wild type (allele G): 104 bp + 25 bp |
| (rs5743708) |
Arg753Gln (allele A): 129 bp |
||
| TLR4 Asp299Gly |
NcoI |
37°C |
Wild type (allele A): 188 bp |
| (rs4986790) |
Asp299Gly (allele G): 168 bp + 20 bp |
||
| TLR4 Thr399Ile |
HinfI | 37°C | Wild type (allele C): 124 bp |
| (rs4986791) | Thr399Ile (allele T): 98 bp + 26 bp |