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. 2013 Mar 28;13:31. doi: 10.1186/1472-6750-13-31

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Copyright © 2013 Ohmuro-Matsuyama et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Comparison with FRET in the presence of a rigid linker (A) SDS-PAGE of the PCA/FRET probes with or without Fn7-8 domains. Lane 1, FKBP/Fn7-8/C; Lane 2, FRB/Ypet; Lane 3, FKBP/Cerulean; Lane 4 FKBP/Fn7-8/Cerulean. (B) FRET assays. Left: the assay using the probe pair without insertion. Right: the assay using the probe pair inserted with Fn7-8. (C) Fluc PCA using the probe inserted with Fn7-8. The probe/rapamycin concentrations of 750 nM (left) and 100 nM (right) were investigated (n = 3).