Fig. 3.

Response surface methodology (RSM), (I–VI) and Factorial Design (V). Box-Behnken showing the effects of: I Glucose-Tween 80; II Glucose-pH and III pH-Tween 80 on Log₁₀ CFUml⁻¹. IV In range optimization of glucose, Tween 80 and pH. V One Factor Design effect of Glucose on Log₁₀ CFU ml⁻¹ maintaining the range optimization of Tween 80 and pH. VI Box-Behnken optimization minimizing the glucose concentration and maintaining Tween 80 and pH within the established range