Table 5.

Carbon, nitrogen and selected inducer optimization

Run order	Point type	Experimental values			Log10 CFU ml−1
		Glucose (g L−1)	Tween 80 (ml L−1)	pH ± 0.2	Observed	Predicted
1	Fact	25 (−1)	0.25 (0)	4.00 (−1)	5.99	6.57
2	Fact	35 (0)	0.00 (−1)	6.00 (+1)	0.00	1.18
3	Fact	35 (0)	0.00 (−1)	6.00 (+1)	6.03	5.94
4	Center	35 (0)	0.25 (0)	5.00 (0)	6.66	6.12
5	Fact	45 (+1)	0.50 (+1)	5.00 (0)	5.67	6.17
6	Fact	45 (+1)	0.00 (−1)	5.00 (0)	5.60	5.00
7	Fact	45 (+1)	0.25 (0)	4.00 (−1)	6.17	6.85
8	Fact	35 (0)	0.50 (+1)	4.00 (−1)	5.98	4.80
9	Fact	25 (−1)	0.25 (0)	6.00 (+1)	6.03	5.94
10	Fact	45 (+1)	0.25 (0)	6.00 (+1)	5.90	5.31
11	Fact	35 (0)	0.50 (+1)	6.00 (+1)	6.03	6.12
12	Fact	25 (−1)	0.50 (+1)	5.00 (0)	5.84	6.44
13	Center	35 (0)	0.25 (0)	5.00 (0)	5.82	6.12
14	Fact	25 (−1)	0.00 (−1)	5.00 (0)	4.31	3.81
15	Center	35 (0)	0.25 (0)	5.00 (0)	5.88	6.12

Box-Behnken. Experiments and results obtained from the response variable (Log₁₀ CFU ml⁻¹)

^aThe observed values of Log₁₀ CFU ml⁻¹ were the mean values of triplicates. Highest values (results) in bold